NAD+ and Senolytics: Laboratory Assessment of Anti-Aging Interventions

NAD+ and Senolytics: Laboratory Assessment of Therapeutic Interventions

Restoring NAD+ (nicotinamide adenine dinucleotide) levels and eliminating senescent cells with senolytics are two of the most promising directions in anti-aging therapy. Clinical trials of NMN, NR (nicotinamide riboside), dasatinib + quercetin and fisetin require objective laboratory monitoring.

For an anti-aging laboratory, this means mastering specific methods for measuring NAD+ and senescence markers, with equipment that partly overlaps with the LC-MS/MS oxidative stress panel.

Methods for Measuring NAD+ and Its Metabolites

Comparison of NAD+/NADH measurement methods:

Markers of Senolytic Therapy Efficacy

Evaluating the efficacy of senolytics requires monitoring SASP factors and markers of cellular senescence:

• SASP factors: GDF15, IL-6, MCP-1, PAI-1. A decrease after a course of senolytics indicates elimination of senescent cells.
• SA-β-galactosidase: cytochemical and fluorimetric determination in skin cells (biopsy) or blood.
• LC3-II/p62: markers of autophagy and mitophagy. Western blot or flow cytometry.
• [Epigenetic clocks](/blog/epigenetic-clocks-biological-age): DunedinPACE and GrimAge as an integrated measure of efficacy.
• [Telomeres](/blog/telomeres-cellular-aging-research-equipment): telomere length as an additional marker of cellular senescence.

Laboratory Equipment

Equipment set for monitoring NAD+ and senolytic therapy:

• LC-MS/MS: the gold standard for NAD+, NADH, NMN and NR. Triple quadrupole with a HILIC column.
• Fluorimeter: Tecan Infinite, BMG CLARIOstar, for enzymatic NAD+ and SA-β-gal assays.
• Flow cytometer: for LC3-II, autophagy and SA-β-gal in cells.
• Western blot station: for LC3-II and p62/SQSTM1. Automated (ProteinSimple Jess/Wes) or conventional.
• Multiplex platform: MSD V-PLEX, Luminex, for simultaneous measurement of a SASP panel.

KombiMED: Equipment for NAD+ and Senolytic Monitoring

KombiMED GmbH supplies equipment for laboratories that monitor NAD+ therapy and senolytic interventions. We will select a configuration that integrates with your existing equipment. Contact KombiMED for a consultation.